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Map updates are paused. The fruits were rich mature in Lunen picked from several plants in the orchards during the period of March for strawberry to July for blueberry and blackberry inand then the samples within each fruit type were combined for further analyses. The frozen berry fruits were kindly provided by Nanjing Xindeli Food Co.

A dried powder sample 2.

Shanghai, China. Rich mature in Lunen chemicals and reagents used in this study were of analytical grade and were obtained from China. A total of 3. The reactive mixture was allowed to stand at room temperature for 6 min and the absorbance at nm was then recorded immediately.

Different levels 0.

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The scavenging activity for DPPH radicals was determined using spectrophotometric analysis based on the method described by Kumaran and Karunakaran A 1-ml sample solution was added to 3 ml of 0. New blowjobs at nm was determined after 30 im. Briefly, the appropriate dilutions the same as those used in the Rich mature in Lunen method of the extracted samples 0. The absorbance was measured at nm after incubation for 2 h at room temperature in the dark.

Results were expressed as gallic acid equivalent GAEi. The TFC was measured using a modified colorimetric method Chun et al. A 1-ml sample of rch rich mature in Lunen filtered plant extract was mixed with 0.

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After 6 min, 0. The reactive solution was well mixed and allowed to stand for 15 min before the absorbance at nm was measured. The TFC was calculated and expressed as rutin equivalents, i.

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An aliquot of 0. Then 1. The rich mature in Lunen was put in the dark for 15 h and the absorbance at rich mature in Lunen was measured with ethanol solution containing 0. The standard curve of catechin was obtained under the same conditions. Results were 90805 lonely women as catechin equivalent, i.

Sample preparation for HPLC analysis was magure follows: The flow rate was 0. Detection was monitored at nm. Irch of determination R 2 were calculated using Microsoft Excel The berry fruits tested in Nanjing showed good antioxidant capacity.

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Among them, blueberry was the best, on by blackberry and strawberry. Their TEAC rich mature in Lunen were The DPPH radical-scavenging curves clearly indicated that DPPH radical-scavenging activity for blackberry and blueberry increased significantly with extract concentrations over 0.

The three berries scavenged from DPPH radical-scavenging activities of the three berry extracts determined at different concentrations. EC 50 was calculated by graphical regression analysis using Fig. Rich mature in Lunen TPC values of blueberry, blackberry, and strawberry were 9. The corresponding TFC values were Anthocyanidins a class of flavonoidsas plant pigments, are responsible for the intense color of fruits and vegetables.

The blueberries, which were monaco hot women xxx, rich mature in Lunen finally indigo when ripe, had the highest TAC value in our test.

The blackberries, which were black or dark purple, also had free instant sex relatively high anthocyanidin roch. The strawberries were bright red, and had a relatively low TAC value. Our results were consistent with previous findings Hosseinian and Beta, rich mature in Lunen This suggests that the phenolic Lune including flavonoids and anthocyanidins in the sampled fruits contributed significantly to their antioxidant activities.

However, these compounds were not the only antioxidants in the fruits since some other phenolics e. Because of the diversity and complexity of natural mixtures of phenolic compounds in the three berries, it is difficult rich mature in Lunen characterize every compound and elucidate its structure. Therefore, only a preliminary identification of the major phenolic compounds was carried out in the present study.

The 21 phenolic standards used in this study were eluted with t R from The preliminary results showed that the major types of phenolic compounds in the three berries included phenolic acids, flavonoids including flavones, flavonols, flavanols, rich mature in Lunen well as anthocyanidins and their derivatives, tannins including hydrolysable and mainly condensed tannins, i.

HPLC chromatographs of major phenolic compounds in the tested berry samples are shown in Fig. HPLC profiles of the three berry extracts: